Eppendorf 5402 Manual ✓

stands as a foundational instrument that bridged the gap between basic sample spinning and the high-precision refrigerated needs of molecular biology. This refrigerated micro-centrifuge was designed specifically for the processing of sensitive biological samples, such as DNA, RNA, and proteins, where temperature stability is critical to preventing degradation. Technical Foundation and Design

| Symptom / Display | Likely Cause | Manual-Recommended Action | | :--- | :--- | :--- | | | Lid not closed; microswitch failure; no power. | Check lid latch. Bypass test: listen for a click. Check fuse on back panel. | | Excessive vibration (noise) | Imbalanced load; rotor not seated; broken damping ring. | Rebalance tubes. Remove rotor, clean shaft, reseat. Inspect rubber cushions. | | Error E1 (digital models) | Overspeed condition or tachometer signal lost. | Turn off for 30 seconds. Restart. If persists, motor encoder is failing. | | Error E3 | Temperature sensor fault (refrigerated models only). | Run only at ambient. Contact service for sensor replacement. | | Lid won’t open after run | Vacuum lock (if refrigerated); mechanical jam. | Wait 2 minutes for pressure equalization. Use emergency lid release (small hole near the hinge – insert a paperclip). | eppendorf 5402 manual